Cellbiological Methodology

Aim

The course gives basic knowledge about how different methods can be used for studying cell and tissue structure and function. After the course the student will be able to:

• explain how cells and tissues should be prepared for further analysis
• for a given cell biological problem to choose appropriate analytical method
• explain the advantages and disadvantages of different analytical methods
• explain the theory behind the chosen preparation method for cells/tissue
• explain the theory behind the chosen method of analysis
• perform a theoretical project included literature search
• account for project work in written and oral
• defend their own presentation and oppose to the work of others Course content Tissue and cell preparation for microscopy.Biological markers used in methods discussed within the course.Fluorometry, cytometry and flow cytometry. Cell culture and cell separation techniques. Chemiluminescence. Electron microscopy, fluorescence microscopy, ratio imaging and confocal microscopy: construction and function of the equipment. Teaching and working methods The course consists of lectures, practical exercises on the basis of problem-based learning as well as a theoretical project and seminars. Examination UPG1Hand-in assignments w oral a written presentation.Opposition3 creditsU, GLAB1Laboratory work with oral and written reports3 creditsU, GGrades are given as ‘Pass’ or ‘Fail’. Grades for examination modules are decided in accordance with the assessment criteria presented at the start of the course. Grades Two grade scale, older version, U, G Other information Supplementary courses: Master profiles in Engineering Biology. About teaching and examination languageThe teaching language is presented in the Overview tab for each course. The examination language relates to the teaching language as follows: If teaching language is “Swedish”, the course as a whole could be given in Swedish, or partly in English. Examination language is Swedish, but parts of the examination can be in English.If teaching language is “English”, the course as a whole is taught in English. Examination language is English.If teaching language is “Swedish/English”, the course as a whole will be taught in English if students without prior knowledge of the Swedish language participate. Examination language is Swedish or English depending on teaching language.OtherThe course is conducted in such a way that there are equal opportunities with regard to sex, transgender identity or expression, ethnicity, religion or other belief, disability, sexual orientation and age.The planning and implementation of a course should correspond to the course syllabus. The course evaluation should therefore be conducted with the course syllabus as a starting point. The course is campus-based at the location specified for the course, unless otherwise stated under “Teaching and working methods”. Please note, in a campus-based course occasional remote sessions could be included. Department Institutionen för biomedicinska och kliniska vetenskaper Course literature Artiklar och kompendier

Content

Tissue and cell preparation for microscopy.Biological markers used in methods discussed within the course.Fluorometry, cytometry and flow cytometry. Cell culture and cell separation techniques. Chemiluminescence. Electron microscopy, fluorescence microscopy, ratio imaging and confocal microscopy: construction and function of the equipment.